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Image Search Results
Journal: eLife
Article Title: Rapid whole brain imaging of neural activity in freely behaving larval zebrafish (Danio rerio)
doi: 10.7554/elife.28158
Figure Lengend Snippet: Figure 2. System schematics that integrated tracking, whole brain functional imaging, and real time behavioral analysis. Larval zebrafish swam in a customized chamber with an optically transparent ceiling and floor. The water-filled chamber was mounted on a high-speed three-axis stage (PI M686 and PI P725KHDS). Customized LED rings generated dark field illumination of the zebrafish. The scattered light was collected by four cameras: two cameras below the chamber were used for x-y plane tracking and low magnification real-time (RT) analysis, respectively; two cameras above the chamber and after the imaging objective were used for Z autofocus and high magnification RT analysis. The positional information of the larval zebrafish, acquired from the tracking and autofocus system, was converted to feedback voltage signals to drive the three-axis stage and to compensate for fish movement. The functional imaging system, described in Figure 1, shared the same imaging objective placed above the swimming chamber. The 3D tracking, RT behavioral analysis, and functional imaging system were synchronized for accurate correlation between neural activity and behavioral output. DOI: https://doi.org/10.7554/eLife.28158.020 The following figure supplement is available for figure 2:
Article Snippet: The
Techniques: Functional Assay, Imaging, Generated, Activity Assay